Physics Colloquium
Friday, November 7th, 2003 4 P.M.
E300 Math/Science Center
Lloyd Davis
Center for Laser Applications,
University of Tennessee Space Institute
Fluorescence Correlation Spectroscopy (FCS) and
Fluorescence Cross-Correlation Spectroscopy (FCCS)
FCS is a resourceful method for determination of the concentrations
and kinetic and photophysical properties of solution constituents
from measurements of fluorescence fluctuations, which arise when
the number of molecules observed at any given time is down to the
single-molecule level. The application of FCS and FCCS to pharmaceutical
drug discovery is discussed. Also, a Monte Carlo simulation of FCS,
which gives added insight into the effects of experimental features
such as saturation, triplet photo-physics, and statistical feature,
is demonstrated.
Refreshments 3:30 P.M. Room E200 Math/Science Center
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